WPI PV microinjectors

Microinjectors

Permeability, Conductivity and Drug studies

Product Description

 

 

Microinjectors For All Your Microinjection Applications

Designed to simplify intracellular injection and a variety of other microinjection tasks, the WPI introduces three new Microinjectors:

Type MICRO-ePUMP
uPUMP
PV850
Regulated compensation and injection pressure
Pressure output: 0.3–87 PSI
Low volume tubing assembly
Inject using foot switch (included)
Inject using a TTL pulse from external computer
or pulse generator (Cable to connect to pulse
generator included)
Inject manually using touch screen interface
Integrated pressure source
2-Stage foot switch to activate voltage for injection
and MICRO-ePORETM
Onboard MICRO-ePORETM

  • Pinpoint Cell Penetrator for targeted microinjection and increased viability of injected embryos
  • Audio continuity tone indicating active probe
  • Injection counter to indicate total number of injections
  • User-adjustable frequency and voltage through touch screen

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New Generation of Pressure Injectors

For All Your Microinjection Needs

WPI’s new generation pressure microinjectors are quickly replacing the very popular legacy line of pnuematic valve PicoPumps Pressure Injectors. The PV820 and PV830 have been a staple in the microinjection market for decades. The three new injectors facilitate any type of microinjecion that involves glass micropipettes.

  • MICRO-ePUMP – Microinjector with Integrated Cell Penetrator and Internal Pressure Source
  • μPUMP – Microinjector with Internal Pressure Source
  • PV850 – Microinjector for Use with External Pressure Source 

One common microinjection method uses a glass micropipette to inject a liquid substance into a living cell or into the intercellular space, typically using an inverted microscope. The glass microinjection needle tip can vary in size from sub-micron to about 5-6µm in diameter. It will be larger if you are injecting stem cells into an embryo. The needles penetrate the cell membrane or even the nuclear envelope, in order to introduce a vector into a cell or nucleus. The vector could be a DNA molecule that carries foreign genetic material into the host cell where it replicates. By altering the embryo, microinjection can be used to genetically modify various animals like mice, fish (zebrafish, medaka, etc.), C. elegans, rats, rabbits, and even large domesitcated animals  like cattle, sheep and pigs.

 

 Benefits WPI Microinjectors Benefits

  • Convenient internal pressure source (MICRO-ePUMP and uPUMP only)
  • Intuitive user-interface
  • Small footprint takes up very little bench space
  • Easy to navigate with touch screen and control knob
  • High precision for reproducible injections – microliters to picoliter
  • All inclusive system
  • Plug-and-play system to begin working as quickly as possible
  • Compensation pressure comparisson for accuracy

    Download Microinjectors Whitepaper Now

Applications

  • Explosive sensing with insect-based biorobots
  • Electron transfer kinetics on mono and multilayer graphene
  • Creation of transgenic animals
    • Any nucleic acid can be introduced into any kind of cell
    • No length limitation (100’s of kb’s of DNA) on the transfer of foreign genes
    • Used to genetically modify animals like mice, fish, C. elegans, rats, rabbits and large domestic animals such as cattle, sheep, and pigsMicroinjectors used to genetically modify animals like mice, fish, c. elegans, rats, rabbits, cattle, sheep, and pigs.
  • Customer QuoteInjecting substances into cells
    • Nucleic acids
    • Organelles
    • Kinases
    • Histochemical markers (such as horseradish peroxidase or fluorescent yellow)
    • Proteins, antibodies
    • Metabolites
    • Ions
  • Microperfusion, which is the precise delivery of a small amount (pL to µL) of drug to a single cell or group of cells, such as a pharmacological drug test
  • Analysis of early development processes like gastrulation, organogenesis, etc.

 

All Your Microinjection Accessories

Let WPI be your one-stop shop for all your microinjection needs. From microscopes and manipulators to pre-pulled glass pipettes, WPI offers many options for your microinjection system.

WPI is your one-stop shop for all microinjection needs. From microscopes and manipulators to pre-pulled glass pipettes, WPI offers many options for your microinjection system.

Options

Order code Integrated Pressure Source Integrated MICRO-ePORE
MICRO-ePUMP Yes Yes
uPUMP Yes No
PV850 No No

Benefits

  • Convenient internal pressure source (MICRO-ePUMP and uPUMP only)
  • Intuitive user-interface
  • Small footprint takes up very little bench space
  • Easy to navigate with touch screen and control knob
  • High precision for reproducible injections – microliters to picoliter

Applications

  • Microinjection of diverse compounds and biomolecules – DNA, RNA, proteins
  • Pre- and post-implantation in embryos of various species – mice, rats, monkeys, bovine, pigs, zebrafish, etc.

 

PV850 Manual

MICRO-ePUMP Manual

uPUMP Manual