REMS
Replacement_REMS_50742840e4bf8_lrg

SYS-REMS

S$45,000.00

Item#: SYS-REMS
  • PC-controlled tissue resistance measurement system
  • Use on transepithelial, trans endothelial, Caco-2 cell membranes
  • Reproducible, accurate, flexible and ease-to-operate
  • Use with 24- and 96-well plates and insert

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Product Description

Automated Tissue Resistance Measuring System

Item#: SYS-REMS
  • PC-controlled tissue resistance measurement system
  • Use on transepithelial, trans endothelial, Caco-2 cell membranes
  • Reproducible, accurate, flexible and ease-to-operate
  • Use with 24- and 96-well plates and insert

The video below demonstrates the REMS robotic automated TEER measurement system and discusses the recent updates made to the software. See the full-size video.

Automated TEER Measuring System

The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates. It is a PC-controlled, tissue resistance measurement system that offers reproducibility, accuracy, flexibility and ease-of-operation for this kind of measurement. Automated measurement of tissue resistance in cell culture microplates provides the important advantages of speed, precision, decreased opportunity for contamination and the instant availability of measured resistance data on a computer. These measurements are useful in applications such as drug bioavailability studies and studies on the mechanisms of drug transport. The main components of the REMS AutoSampler include: the robotic sampler that moves the electrode over each well of the microplate, the electrode which is located on the robotic arm, a base plate for the 24- and 96-well tray, a Windows-based data acquisition card, the REMS interface unit and the REMS software to operate the system on a Windows-based computer.

Automatically measure and record tissue resistence

The REMS AutoSampler automates TEER measurements previously made with WPI’s EVOM2 Epithelial Voltohmmeter. Automated tissue resistance measurements up to 20 ohm can be performed on 24- or 96-well HTS microplates. Microplates presently supported include the Corning Costar HTS Transwell-24, Falcon HTS Multiwell insert systems, and Millipore Multiscreen™ CaCo 96-well plate. The REMS AutoSampler is designed to facilitate integration with other robotic systems. Special locating bars are installed on the REMS base platform that allow other system robots to place an HTS tray into a precise location on the REMS base. The REMS AutoSampler will automatically measure and record tissue resistance from a user-specified matrix of culture wells on the microplate. According to the specified sequence, the robotic arm moves over the identified wells taking TEER measurements. By means of a x-y-z locating system, the electrode-containing arm is positioned precisely and reproducibly over each well. The ability of the REMS AutoSampler to reproducibly and precisely locate the electrode results in highly reproducible TEER measurements. TEER measurements are stored in the computer as the electrode moves from one well to the next. The Windows-based software provides user-friendly features to acquire, display and store the tissue resistance measurements.

Compact, robust electrode

The REMS electrode is very compact and robust in design. Each of two rod-shaped probes, 1.5 mm in diameter, consists of a pair of electrodes: one electrode for injecting current and the other for measuring the voltage. The use of two pairs of electrodes eliminates the error caused by the electrode-liquid interface. To take a measurement, the robot inserts one probe into the center of the filter well and the other into the opening slot of the 24- or 96-well plate. The use of AC current to measure resistance provides several advantages over DC current, including:

  • absence of offset voltages on measurements
  • there is a zero net current being passed through the membrane and, therefore, it is not adversely affected by a current charge
  • no electrochemical deposition of electrode metal

Rinse and calibration station

The REMS AutoSampler also features a rinse and calibration check station. If occasional rinsing of the REMS electrode is required it may be sent to a rinse station by pressing the rinse station button on the menu bar. The rinse station can also function as a calibration check station when fitted with a calibration cell containing a synthetic membrane. The use of this calibration cell, which mimics a confluent epithelial membrane’s resistance in fluid, provides a quick and effective test to determine when the REMS measurement system is fully initialized following start-up and to check its functionality during operation.

The following plates can be used with the REMS system:

REMS-24 REMS-24 REMS-96 REMS-96 REMS-96C
Corning HTS Transwell BD Falcon HTS MultiWell Millipore HTS96 Multiwell BD Falcon Corning HTS Transwell
3378
3379
3396
3397
3398
3399
351180
351181
351182
351183
351184
351185
354803
354804
PSHT004R1
PSRP004R1
PSHT010R5
PSRP004R5
PSHT004S5
351130
351131
351161
351162
351163
351164
3380
3392
3381
3391
3385
3386
3387
3388
3374
3384

Sample Plates

Corning #3378 24-well plate
Click to enlarge.
BD Falcon 96-well plate
Click to enlarge.
Millipore Multiscreen CaCo2
Click to enlarge.
Corning 3391 HTS Traswell 96 plate
Click to enlarge.
This is a Corning #3378 24-well plate that may be used with the REMS-24 or the STX100C.

The BD Falcon 24-well plate is virtually identical to this one. For use with REMS-24 or STX100F.

 This is a BD Falcon 96-well plate that may be used with the REMS-96.  This is a Millipore Multiscreen CaCo2 that may be used with the REMS-96 or the STX100M.  This is a Corning 3391 HTS Traswell 96 plate that may be used with the REMS-96C or the STX100C96.
MEMBRANE RESISTANCE RANGE 0 to 2000Ω and 0 to 20kΩ
AC SQUARE WAVE CURRENT +/- 20μA @ 12.5 Hz
ELECTRODE POSITIONING Resolution in X, Y and Z: +/- 1mm
ELECTRODE PERFORMANCE Repeatability in X, Y and Z: +/- 0.25mm
ELECTRODE ARM SPEED X- and Y-axis: 250mm/sec Z-axis: 247.3mm/sec
TYPICAL MEASUREMENT TIME 24-WELL 1 min, 10 sec
SCAN PATTERN Choice of any well pattern sampling
LINE VOLTAGE User specified: 100/120 V or 220/240 V
DIMENSIONS 53.5 x 43.7 x 37.1 cm (21.09 x 17.19 x 14.63 in.)
WEIGHT 24 kg (52 lb)

The system includes robot sampler, base plate, data acquisition board, computer, monitor, keyboard, mouse, software for Windows 7 or Windows 8, and electrode for either 24-well plate (Corning Costar HTS Transwell-24 or Falcon HTS Multiwell) or 96-well plate (Millipore Multiscreen CaCo or Corning 96). Please specify which plate when ordering.

REMS is supplied with a desktop computer. The 2015 computer shipping is an I5, quad core 3.3 GHz CPU with 4 GB RAM and a 500Gb hard drive, a 16:9 monitor, US standard keyboard and a mouse.

 

 

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